Determination of cytokine gene polymorphisms in Turkish patients with multiple myeloma
Çiğdem Kekik*, Sevgi Beşışık**, Fatma Savran Oğuz*, Yalçın Seyhun*, Gonca Emel Karahan*, Mehmet Gürtekin*, Deniz Sargın**, Mahmut Çarin*
*Department of Medical Biology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
**Department of Hematology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
Cytokines play a major role on multiple myeloma (MM) pathogenesis. Polymorphisms occurring in cytokine genes are effective on plasma level and/or function of the protein. The aim of our study was to determine the cytokine gene polymorphism (CGP) in patients with MM. Eighty patients with MM and 150 healthy individuals were included in the study. CGPs were typed by PCR method using sequence-specific primers (SSP) with Heidelberg kit. TGF-beta CG/CC, TNF-alpha AG/AA, IL-2 GG/TT and IL-4 GCC/TCC haplotypes in the patient group and TNF-alpha GG/GG haplotype in the control group were found to be significantly frequent. When the cytokines that have more than one polymorphic site were investigated one by one CC genotype of IL-4 gene at position -590 was found to be significantly frequent in the patient group. For TGF-beta, CC genotype at codon 10 and GC genotype at codon 25; for TNF-alpha, AA genotype at position -308 and GA genotype at position -238; for IL-2 gene, GT genotype at position +166 were found to be significantly frequent in the patient group. We believe that further studies regarding cytokine polymorphisms in MM may clarify the role of cytokines and contribute to identify novel therapeutic targets.