Adv Mol Med 2007; 3(4): 183-188

Published online: June 7, 2008
DOI 10.2399/amm.07.183

Protective effects of tomato consumption against the oxidative damage caused by CCl4 in rat's liver


Tuncay Altuğ*, Seval Aydın**, Elif İlkay Taşkın*, Kadriye Akgün Dar***, Metin Caner****, Ahmet Belce**


*Department of Veterinary Pre-science, Veterinary Medicine Faculty, Istanbul University, Istanbul, Turkey
**Department of Biochemistry, Istanbul Medicine Faculty, Istanbul University, Istanbul, Turkey
***Department of Biology, Science Faculty, Istanbul University, Istanbul, Turkey
****Department of General Surgery, Cerrahpasa Medicine Faculty, Istanbul University, Istanbul, Turkey


Objective: The aim of the study was to examine the effects of tomato juice, which is as a rich source of lycopene, on acute liver injury caused by the oxidant CCl4.

Background: Lycopene is the mostly encountered carotenoid in Lycospersicum esculentum. Like tomato, pink guava, papaya and apricot also involve lycopene which is a more effective antioxidant than the other carotenoids. CCl4 is a xenobiotic that causes hepatotoxicity. In daily life it is used as organic solvent in oil products, insecticides, resin, wax, organic diluent in rubber products and as a transfer agent in cooling equipments. Because of its solvent property it is used as stain remover for furniture and carpets, as well as an anesthetic in extraction of plants. Because of these reasons CCl4 is contagious to human by breathing, swallowing and contact.

Methods: 26 Wistar albino rat were separated into 3 groups; To Group I (Control group) physiologic serum equal to the amount of CCl4 volume administered to other groups’ animals was injected, To Group II (CCL4 Group) CCl4 was injected to the animals, to Group III (CCL4+Lycopene) CCl4 was injected to the animals, and only to the animals of this group, starting 20 days before the first administration of CCl4 and throughout the study period, 12.5 ml. of squeezed fresh tomato juice was given daily by their water bottles throughout the study. At the end of the study animals were sacrificed and tissue samples from their liver were investigated to establish MDA, SOD, GSH, GPx values.

Results: MDA levels were higher in CCl4 administered group than the control group; in the group which received tomato juice, those levels were close to the control group. In the group which received CCl4 + tomato juice SOD, GSH and GPx enzyme values were meaningfully higher when compared with the group which only received CCl4 (For CCl4 + tomato juice group P<0.0001). Those three enzyme levels were statistically significant when compared to the control group and CCl4 + tomato juice receiving group 3. (P<0.05, P<0.0001, P?0.01 respectively).

Conclusion: According to the result of our study, tomato juice containing lycopene and ascorbic acid, which have antioxidant properties, possess a strong effect on oxidative damage of CCl4 in liver.

Key words

Tomato juice, carbon tetrachloride, rat, liver, antioxidant

Adv Mol Med 2007; 3(4): 183-188

Advances in Molecular Medicine, official journal of the Turkish Society of Molecular Medicine, is published by Deomed Medical Publishing, Istanbul.
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